Autoimmune disease diagnostic technologies
LIA Technology
Autoimmune diseases are characterized by autoantibody patterns found in patient sera. HUMAN’s autoimmune line immuno assays (LIAs) are developed for rapid autoantibody profiling. LIAs are simple to use screening assays. By employing disease-specific antigens, up to 18 different autoantibodies can be differentiated with a single-shot assay. This state-of-the-art autoimmune diagnostic tool is produced by spotting antigens in lines on a simple nitrocellulose membrane utilizing the newest technologies available in the market, including HUMAN’s patented epitope-preserving technology.
Bound autoantibodies are visualized by using specific HRP-conjugated secondary antibodies that specifically target human antibodies and a LIA specific substrate. Samples positive for autoantibodies can be easily detected by comparing the pattern of brown lines to the standardized line (cut-off) that is also spotted on the LIAs. LIAs are ideal autoimmune diagnostic tools for screening and differential diagnosis as well as monitoring autoimmune patients by identifying specific autoantibodies in one shot.
ELISA Technology
The HUMAN ELISAs for Autoimmune DX offer a unique solution for all sizes of laboratories from low to high throughput. The ELISA line consists of around 60 ELISAs (both qualitative and quantitative) for the differential diagnosis of autoimmune diseases. To provide the best solution for the requirements of each laboratory, HUMAN offers screening ELISAs for the detection of different subtypes (IgG, IgM, IgA) in one assay and single-parameter ELISAs for separate autoantigens. The quantitative ELISAs are ideally suited for the monitoring of disease progression. All ELISAs are optimized for automation and most are validated for use with HUMAN’s automated ELISA instruments: Elisys Uno, Elisys Duo and Elisys Quattro. Handling is as simple as can be thanks to ready-to-use reagents, breakable microtiter wells and reagents largely interchangeable between different assays and lots. The incubation conditions are the same for almost all assays.
Rapid Screening Technology
HUMAN’s autoimmune rapid screening tests use latex beads coated with disease relevant and specific antigens. In the presence of autoantibodies in patient serum, the latex beads agglutinate and provide results in less than 2 minutes. Rapid tests for screening are ideal for identifying potential autoimmune patients with rheumatic diseases quickly and easily.
